Cre-LoxP System

Cre-LoxP site-specific recombination system is widely used to carry out genomic editing, including deletion, insertion, translocation and inversion both in eukaryotic and prokaryotic species. The system consists of two components: Cre recombinase and DNA sequences named LoxP site. Cre recombinase is a 38kDd protein encoded by bacteriophage P1. It cooperates to loxP sites by contacting each of two 13bp recombinase binding elements (RBE) arranged as inverted repeats flanking a central asymmetric 8bp strand-exchange region, which in combination form the LoxP site. The asymmetric 8bp sequence is the only factor that contributes to the orientation of LoxP site.

Cre binding to the loxP sites (Gregory D. Van Duyne .Annu. Rev. Biophys. Biomol. Struct. 2001. 30:87–104 )

The system usually works in the following ways:
● Two LoxP sites with the same orientation leads to the deletion of the sequences between these two sites,
● Two LoxP sites with an opposite orientation leads to an inversion of the sequences between them,
● Two LoxP sites in transfacilitatesDNA insertion with the aid of Cre recombinase.

Cre/loxP system. ( Sabine L. Neurogenetics (2011) 12:97–116 )

BrainVTA offers adeno-associated virus (AAV) services with the Cre-LoxP site-specific recombination system, please visit the pre-made AAV center to check the pre-made tools.
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References
1. Sabine L. Renninger & Helia B. Investigating the genetics of visual processing, function and behaviour in zebrafish .Neurogenetics (2011) 12:97–116.
2. Gregory D. Van Duyne.A STRUCTURAL VIEW OF Cre-loxP SITE-SPECIFIC RECOMBINATION .Annu. Rev. Biophys. Biomol. Struct. 2001. 30:87–104.
3. Ying-Jie Yang, Raghvendra Pratap Singh. Genome Editing in Model Strain Myxococcus xanthus DK1622 by a Site-Specific Cre/loxP Recombination System .Biomolecules 2018, 8, 137.
4. BRANDON C. COX, ZHIYONG LIU, MARCIA M. MELLADO LAGARDE.Conditional Gene Expression in the Mouse Inner Ear Using Cre-loxP. JARO 13: 295–322 (2012).
5. Manuel Aranda, Chryssa Kanellopoulou. Altered Directionality in the Cre-loxP Site-specific Recombination Pathway .J. Mol. Biol. (2001) 311, 453–459.
6. Micheal A. McLellan .Cre-loxP-Mediated Recombination:General Principles and Experimental Considerations Current Protocols in Mouse Biology 7:1-12, March 2017.
7. Bo Young Nam,Dong Ki Kim, Jung Tak Park.Double transduction of a Cre/LoxP lentiviral vector: a simple method to generate kidney cell-specific knockdown mice .Am J Physiol Renal Physiol 309: F1060–F1069, 2015 .