Pseudorabies virus(PRV)

Pseudorabies virus(PRV) have a double-stranded DNA genome, and the genome is approximately 150 kb that encode capsid, tegument, and envelope protein. The retrograde trans-synaptic PRV is modified from vaccine strain Bartha, which can be used to analyze the structure of neuronal connectivity in brain center and periphery. After the virus infects nerve cells, it can replicates and expresses the target gene in the cell. The progeny viruses are transported to the synapse and then retrograde trans-synaptic labeling of the upstream neuron for further replication and transmission across the synapse.

After years of research, BrainVTA has established a stable production platform, and could offer pseudorabies virus(PRV) services to promote PRV-based neuronal tracing studies.
Applications
● Multisynaptic tracing from the peripheral to brain center, or the higher to the lower brain region.
● Tracking peripheral organs' connections to the central nervous system
● Tracking the neural networks in disease or injury model.
● Changes in neural networks during neural development.

Popular PRV particles

Cat#	Name	Description
P01001	PRV-CMV-EGFP	Retrograde,multisynaptic
P01002	PRV-CMV-RFP	Retrograde,multisynaptic
P01003	PRV-hUbC-EGFP	Retrograde,multisynaptic
P03001	PRV-CAG-EGFP	Retrograde,multisynaptic

Successful case

1. Wangxiaojun. Plastic embedding immunolabeled largevolume samples for three-dimensional highresolution imaging. doi: 10.1364/BOE.8.003583.
2. Zhijian Zhang, Hongruo Zhang, Pengjie Wen, Xutao Zhu, Li Wang, Qing Liu, Jie Wang, Xiaobin He, Huadong Wang and Fuqiang Xu. Whole-Brain Mapping of the Inputs and Outputs of the Medial Part of the Olfactory Tubercle. https://doi.org/10.3389/fncir.2017.00052.
3. Hao-LongZeng, Fei-LongYu,ZhijianZhang, QingYang, SenJin, XiaobinHe. Quantitative proteomics study of host response to virulent and attenuated pseudorabies virus infection in mouse brain. https://doi.org/10.1016/j.bbapap.2017.11.010.
4. Haixia Liu; Xutao Zhu; Yun Ling; Xiaobin He; Lei Pei; Zhidan Zhang; Fang Yang; Fuqiang Xu. Corticosterone signaling and a lateral habenula-ventral tegmental area circuit modulate compulsive self-injurious behavior in a rat model.https://doi.org/10.1167/iovs.18-24308.

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