Lentivirus (LVs) are single stranded RNA (sRNA) viruses, which are a subgroup of the retrovirus family. They can integrate into the host genome in both dividing and non-dividing cells, with a wide range of cell, resulting in long-term expression both in vitro and in vivo. The delivery vehicles based on HIV-1 have been developed by BrainVTA.
The advantages of LV compared to other viral vectors
● Long-term gene expression: Lentivirus can achieve long-term stable expression of target genes by integrating genes into the host cell genome, and will not be lost with cell division, which is the first choice for cell experiments.
● High safety. No pathogenicity has been found, and it has been used in CAR-T therapy in human;
● Low immunogenicity. Direct injection of tissues in vivo is not easy to cause an immune response and is suitable for animal experiments.

Viral vector	AAV	LV	Ad
Genome	ssDNA	ssRNA (+)	dsDNA
Coat	Naked	Enveloped	Naked
Type	Non-integrating	Integrating	Non-integrating
Infection	Dividing and non-dividing cells	Dividing and non-dividing cells	Dividing and non-dividing cells
Packaging Capacity	4.7kb	6kb	7.5kb
Transgene expression	Potentially long-lasting	Long-lasting	Transient
Immune Response	Very Low	Low	High
Titer	Up to 1012-13v.g/ml	Up to 109TU/ml	Up to 1012pfu/ml
Expression abundance	High-level expression	Moderate to high level expression	High-level expression
Safety	No pathogenicity has been found yet, Has been approved by the EU and FDA, Used as a carrier for gene therapy drugs	No pathogenicity has been found yet, It has been used in CAR-T therapy in the human	May cause some coughing and runny nose

How do I obtain virus packaging services?
● You can click Pre-Made LVs to find out whether the list contains what you need.
● Also, You can obtain custom-Made LVs service by clicking here.

Characteristics of (HIV) Based Vectors 
● Infects dividing and non-dividing cells;
● Long term, stable gene expression resulting from genomic integration;
● Pseudotyping with a VSV-G envelope glycoproteins that broaden cell tropism;
● Factors such as expression cassette size or choice of envelope will influence titer;
● HIV accommodates insert sizes of 6.5 kb. Larger expression cassettes can be packaged but decreases in titers will be expected.

Selection of vectors
BrainVTA provides a complete lentivirus product system, which can manipulate coding and non-coding genes, such as lncRNA, microRNA, and circRNA. For more details, please contact sales@brainvta.com

Ready to order?
Further information about the lentiviral vectors, just click the Pre-Made LVs  or the Custom-Made LVs Button and submit your needs to us.