Vesicular Stomatitis Virus（VSV）

Vesicular stomatitis virus (VSV) is a non-segmented, negative-strand RNA virus in the genus of Vesiculovirus of Rhabdoviridae family. The genome of VSV is approximately 11kb-12kb nucleotides which encodes nucleoprotein (N), phosphoprotein (P), matrixprotein (M), glycoprotein (G) and large polymerase protein (L). The modified VSV has been widely used as an anterograde tracer.
Glycoprotein (G) is an essential component for VSV to anterograde transsynaptic. When removed G (VSV-∆G), it will lose the ability to cross synapses, but still can express the foreign gene in high abundance. Therefore,VSV-∆G can be used to quickly obtain a complete image of the projection distribution of neurons, and can also be used for anterograde monosynaptic tracing.

BrainVTA has established the recombinant VSV platform based on our experienced staffs and our advanced molecular biology techniques, and could offer VSV services to promote VSV-based neuronal tracing studies.
Applications
● Multisynaptic tracing from peripheral to brain center, or the lower to the higher brain region.
● Tracking the neural networks in disease or injury model.
● Changes in neural networks during neural development.

Popular VSV particles

Cat#	Name	Description
V01001	VSV-eGFP	Anterograde,multisynaptic
V01002	VSV-mCherry	Anterograde,multisynaptic
V01003	VSV-BFP	Anterograde,multisynaptic
V02001	VSV-EnVA-ΔG-eGFP	Combined with AAV-TVA and AAV-VSVG, anterograde monosynaptic
V02002	VSV-EnVA-ΔG-mCherry	Combined with AAV-TVA and AAV-VSVG, anterograde monosynaptic
V03001	VSV-ΔG-eGFP	Combined with AAV-VSVG, anterograde monosynaptic
V03002	VSV-ΔG-mCherry	Combined with AAV-VSVG, anterograde monosynaptic
V03003	VSV-ΔG-taueGFP	Combined with AAV-VSVG, anterograde monosynaptic
V03004	VSV-ΔG-taueGFP-ferritin	Combined with AAV-VSVG, anterograde monosynaptic

References
1. Hastie E, Grdzelishvili VZ. Vesicular stomatitis virus as a flexible platform for oncolytic virotherapy against cancer. J Gen Virol. 2012 Dec;93(Pt 12):2529-45.
2. Bishnoi, S., Tiwari, R., Gupta, S., Byrareddy, S.N., Nayak, D. Oncotargeting by Vesicular Stomatitis Virus (VSV): Advances in cancer therapy. Viruses. 2018; 10(2): 90.
3. Beier KT, Saunders A, Oldenburg IA, Miyamichi K, Akhtar N, Luo L, Whelan SP, Sabatini B, Cepko CL. Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9.

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