COVID-19 Coronavirus Research Tools
Pre-Made AAVs Center
Neuronal Tracing Tool

BrainVTA

Construction and Packaging Service of virus Vector

Construction and Packaging Services of AAV

Large scale AAV production

Optical stimulation protocol
Optogenetics provides a powerful approach for investigating neuronal electrophysiology. Take the light stimulation of the wireless optogenetic control system as the example, the operating procedures of optical stimulation is as follows.

Image source:McGovern Institute for Brain Research at MIT
STEP 1:
The wireless optogenetic control system performs bluetooth pairing through a receiver (weight 3.7 g,  length × width × height 16 mm × 15.6 mm × 10.8 mm) connected with the LED, so as to achieve the purpose of remote light control of specific neuron activity.
STEP 2:
Light stimulation parameters: 473 nm, 5.0 mW, 20 Hz, 25 ms blue light stimulation is used to activate glutamatergic neurons in bilateral VLO; 473 nm, 5.0 mW continuous blue light stimulation is used to inhibit GABAergic neurons in bilateral VLO.
STEP 3:
The light duration is 3 min. In the behavioral experiment for 6 minutes, there was no light in the first 3 minutes and light in the last 3 minutes.

If you have any questions,please email us at [email protected].




50% discount for Pre-Made AAVs