Optogenetics provides a powerful approach for investigating neuronal electrophysiology. Take the light stimulation of the wireless optogenetic control system as the example, the operating procedures of optical stimulation is as follows.
Image source：McGovern Institute for Brain Research at MIT
STEP 1:
The wireless optogenetic control system performs bluetooth pairing through a receiver (weight 3.7 g, length × width × height 16 mm × 15.6 mm × 10.8 mm) connected with the LED, so as to achieve the purpose of remote light control of specific neuron activity.
STEP 2:
Light stimulation parameters: 473 nm, 5.0 mW, 20 Hz, 25 ms blue light stimulation is used to activate glutamatergic neurons in bilateral VLO; 473 nm, 5.0 mW continuous blue light stimulation is used to inhibit GABAergic neurons in bilateral VLO.
STEP 3:
The light duration is 3 min. In the behavioral experiment for 6 minutes, there was no light in the first 3 minutes and light in the last 3 minutes.
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