Intravenous injection is a clinically applicable manner to deliver therapeutics. For adult rodents and larger animals, intravenous injections are technically feasible and routine. In this protocol, we take the temporal vein injection as an example to present the experimental procedure in the mouse as follows. Preparation
●Place the mouse directly on the wet ice for 30-60 sec to anesthetize the animal for injection.
●While the animal is on ice, load the syringe with solution to be injected.
●When the animal is fully anesthetized, confirmed by lack of movement on the ice while still breathing, move it under the microscope.
Target coordinates and injection
●Examine just anterior to the ear bud for a superficial capillary that moves when the skin is manipulated. This capillary is not the target, However, it is important for temporal vein identification. Next, locate a dark, shadowy vein inferior to the capillary that remains fixed regardless of skin position. The temporal vein appears shadowy, runs dorsal to ventral, and feeds into the jugular vein.
●Enter the temporal vein with the needle bevel up. If correctly inserted, it is possible to view the needle bevel fill with blood through the skin.
Then depress the plunger slowly and note blanching of the vein down the side of the face.
Post- surgery
●Allow the needle to remain within the vein for an added 10-15 sec to prevent backflow of the solution.
●Return the mouse to its cage until it recovers.
Reference
For more details, please refer to the article "Intravenous Injections in Neonatal Mice".

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