VIP interneurons regulate olfactory bulb output and contribut

AAV-GCaMp6s was used for ﬁber photometry experiment. AAV-hChR2 was used for optogenetic manipulation. AAV-hM4D(Gi) was used for chemogenetics manipulation (From BrainVTA)

The viruses used in this article from BrainVTA are in the table below

Optogenetic	PT-0001 rAAV-EF1a-DIO-hChR2(H134R)-eYFP
Calcium sensors	PT-0071 rAAV-EF1a-DIO-GCaMp6s
Chemogenetics	PT-0043 rAAV-Ef1a-DIO-hM4D(Gi)-mCherry
Control	PT-0012 rAAV-Ef1a-DIO-eYFP

Dejuan Wang, Jing Wu, Penglai Liu, Xiaowen Li, Jiaxin Li, Miao He, Anan Li
Pub Date: 2022-02-15, DOI: 10.1016/j.celrep.2022.110383, Email: sales@brainvta.com

In the olfactory bulb (OB), olfactory information represented by mitral/tufted cells (M/Ts) is extensively modulated by local inhibitory interneurons before being transmitted to the olfactory cortex. While the crucial roles of cortical vasoactive-intestinal-peptide-expressing (VIP) interneurons have been extensively studied, their precise function in the OB remains elusive. Here, we identify the synaptic connectivity of VIP interneurons onto mitral cells (MCs) and demonstrate their important role in olfactory behaviors. Optogenetic activation of VIP interneurons reduced both spontaneous and odor-evoked activity of M/Ts in awake mice. Whole-cell recordings revealed that VIP interneurons decrease MC firing through direct inhibitory synaptic connections with MCs. Furthermore, inactivation of VIP interneurons leads to increased MC firing and impaired olfactory detection and odor discrimination. Therefore, our results demonstrate that VIP interneurons control OB output and play critical roles in odor processing and olfactory behaviors.

In this study, the authors identify a population of VIP interneurons that form monosynaptic GABAergic inhibitory connections with mitral cells in the olfactory bulb and play critical roles in odor processing and olfactory behaviors.

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