An mPOA-ARCAgRP pathway modulates cold-evoked eating behavior

AAV tracing helper and RV were used for cell-type-speciﬁc retrograde tracing to identify the upstreaminput of ARC^AgRP neurons. AAV -hM4D(Gi) was used for chemogenetics manipulation (From BrainVTA)

The viruses used in this article from BrainVTA are in the table below

Chemogenetics	PT-0043-AAV2/9-Ef1a-DIO- hM4D(Gi)-mCherry-WPRE-pA
AAV tracing helper	PT-0021-AAV2/9-Ef1a-DIO-His-EGFP-2A-TVA-WPRE-pA PT-0023-AAV2/9-Ef1a-DIO-RVG-WPRE-pA
Control	PT-0013-AAV2/9-Ef1a-DIO-mCherry-WPRE-pA
RV	R01002-RV-EnvA-ΔG-dsRed

Shuo Yang, Yu Lin Tan, Xiaohua Wu, Jingjie Wang, Jingjing Sun, Anqi Liu, Linhua Gan, Bo Shen, Xiaocui Zhang, Yu Fu, Ju Huang
Pub Date: 2021-08-10, DOI: 10.1016/j.celrep.2021.109502, Email: sales@brainvta.com

Enhanced appetite occurs as a means of behavioral thermoregulation at low temperature. Neural circuitry mediating this crosstalk between behavioral thermoregulation and energy homeostasis remains to be elucidated. We find that the hypothalamic orexigenic agouti-related neuropeptide (AgRP) neurons in the arcuate nucleus (ARC) are profoundly activated by cold exposure. The calcium signals in ARC^AgRP neurons display an immediate-response pattern in response to cold stimulation. Cold-responsive neurons in the medial preoptic area (mPOA) make excitatory synapses onto ARC^AgRP neurons. Inhibition of either ARC^AgRP neurons or ARC-projecting mPOA neurons attenuates cold-evoked feeding, while activation of the mPOA-to-ARC projection increases food intake. These findings reveal an mPOA-ARC^AgRP neural pathway that modulates cold-evoked feeding behavior.

In this study, the authors reveal that ARC^AgRP neurons are profoundly activated in cold exposure and discover an mPOA-ARC^AgRP neural pathway that regulates cold-evoked feeding behavior. This study furthers our understanding of the neural circuitry that bridges the connection between
behavioral thermoregulation and energy homeostasis.

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