Distinct Characteristics of Odor-evoked Calcium and Electro-p

AAV-GCaMp6s was used to simultaneously record odor-evoked Ca²⁺ signals and electrophysiological signals from M/Ts. (From BrainVTA)

The viruses used in this article from BrainVTA are in the table below

Calcium sensors

PT-0071 rAAV-EF1α-DIO-GCaMp6s-WPRE-hGH polyA

Han Xu, Chi Geng, Xinzhong Hua, Penglai Liu, Jinshan Xu, Anan Li
Pub Date: 2021-04-15, DOI: 10.1007/s12264-021-00680-1, Email: sales@brainvta.com
Fiber photometry is a recently-developed method that indirectly measures neural activity by monitoring Ca²⁺ signals in genetically-identiﬁed neuronal populations. Although ﬁber photometry is widely used in neuroscience research, the relationship between the recorded Ca²⁺ signals and direct electrophysiological measurements of neural activity remains elusive. Here, we simultaneously recorded odor-evoked Ca²⁺ and electrophysiological signals [single-unit spikes and local ﬁeld potentials (LFPs)] from mitral/tufted cells in the olfactory bulb of awake, head-ﬁxed mice. Odors evoked responses in all types of signal but the response characteristics (e.g., type of response and time course) differed. The Ca²⁺ signal was correlated most closely with power in the b-band of the LFP. The Ca²⁺ signal performed slightly better at odor classiﬁcation than high-c oscillations, worse than single-unit spikes, and similarly to b oscillations. These results provide new information to help researchers select an appropriate method for monitoring neural activity under speciﬁc conditions.

Figure 1. Simultaneous recording of odor-evoked spikes, LFPs, and population Ca²⁺ signals from M/Ts.

The study is aimed to explore the relationship between the recorded Ca²⁺ signals and direct electrophysiological measurements of neural activity. In this study, the authors used a mouse model with M/T-speciﬁc GCaMP6s expression to simultaneously record odor-evoked Ca²⁺ signals and electrophysiological signals from M/Ts in awake, head-ﬁxed mice. The ﬁndings show how Ca²⁺ signals recorded by ﬁber photometry relate to electrophysiological recordings and have implications for the application of ﬁber photometry throughout the brain.

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