Monosynaptic RV(From BrainVTA) was used to label the whole-brain inputs to specific cell types in the Mop and MOs simultaneously.

Tracing Helper	PT-0095 rAAV2/9-Ef1a-DIO-BFP-2a-TVA-WPRE-pA  PT-0023 rAAV2/9-Ef1a-DIO-RG-WPRE-pA
RV	R01001 RV-MG-EnVA-EGFP

Pan Luo, Anan Li, Yanxiao Zheng, Yutong Han, Jiaojiao Tian, Zhengchao Xu, Hui Gong and Xiangning Li
Pub Date: 2019-04-17,  DOI: 10.3389/fnana.2019.00044， Email: [email protected]
Long-range neuronal circuits play an important role in motor and sensory information processing. Determining direct synaptic inputs of excited and inhibited neurons is important for understanding the circuit mechanisms involved in regulating movement. Here, we used the monosynaptic rabies tracing technique, combined with fluorescent micro-optical sectional tomography, to characterize the brain-wide input to the motor cortex (MC). The whole brain dataset showed that the main excited and inhibited neurons in the MC received inputs from similar brain regions with a quantitative difference. With 3D reconstruction we found that the distribution of input neurons, that target the primary and secondary MC, had different patterns. In the cortex, the neurons projecting to the primary MC mainly distributed in the lateral and anterior portion, while those to the secondary MC distributed in the medial and posterior portion. The input neurons in the subcortical areas also showed the topographic shift model, as in the thalamus, the neurons distributed as outer and inner shells while the neurons in the claustrum and amygdala were in the ventral and dorsal part, respectively. These results lay the anatomical foundation to understanding the organized pattern of motor circuits and the functional differences between the primary and secondary MC.