Long-term Fiber Photometry for Neuroscience Studies

AAV -GCaMP6m was used to monitor the activity of neurons. AAV –mGFP was used for control.

The viruses used in this article are in the table below

Control	AAV -DIO-mGFP
Calcium sensors	AAV -DIO-GCaMP6m

Pub Date: 2019-05-06,DOI: 10.1007/s12264-019-00379-4， Email: sales@brainvta.com
Yi Li • Zhixiang Liu • Qingchun Guo • Minmin Luo
Fiber photometry is a sensitive and easy way to detect changes in ﬂuorescent signals. The combination of ﬁber photometry with various ﬂuorescent biomarkers has substantially advanced neuroscience research over the last decade. Despite the wide use of ﬁber photometry in biomedical ﬁelds, the lack of a detailed and comprehensive protocol has limited progress and sometimes complicated the interpretation of data. Here, we describe detailed procedures of ﬁber photometry for the long-term monitoring of neuronal activity in freely-behaving animals, including surgery, apparatus setup, data collection, and analysis.

Fig. 1 The ﬁber photometry system.

In this article, the authors describe detailed procedures of ﬁber photometry for the long-term monitoring of neuronal activity in freely-behaving animals, including surgery, apparatus setup, data collection, and analysis.

BrainVTA offers viral vector construction & virus packaging services for AAV, LV, RABV, PRV, HSV and VSV that help researchers explore questions about genes, neurons, circuitry structure, function of brain network, mechanism and treatment of diseases.
If you have any needs, just email us at sales@brainvta.com.