ChR2-mCherry (From
BrainVTA) was used for optogenetics to manipulate the activity of PVC interneurons, hM3Dq -mCherry and hM4Di –mCherry were used for an enhancement of neuronal excitability and suppression of neuronal firing, respectively.
The viruses used in this article from BrainVTA are in the table below
|
Control |
PT-0013 AAV9-EF1a-DIO-mCherry |
|
Optogenetic |
PT-0002 AAV9-EF1a-DIO-ChR2-mCherry |
Xi Chen, Zhiyuan Liu, Chaonan Ma, Lan Ma and Xing Liu
Pub Date: 2019-05-14,
DOI: 10.3389/fnbeh.2019.00110，
Email: [email protected]
Parvalbumin (PV) expressing GABAergic interneurons provide large source of GABA to spiny projection neurons (SPNs) in the striatum. However, the roles of PVC interneurons in the regulation of SPNs in the ventral striatum and emotional states are largely unknown. Here, we investigated whether stimulation of ventral striatal (accumbal) PVC interneurons would drive emotional valence in mice. We found that during conditioned place preference (CPP) training, activation of accumbal PVC interneurons evoked place preference while suppressing them resulted in conditioned place aversion (CPA). Activation of PVC interneurons during place conditioning increased Fos expression in SPNs in the direct pathway (dSPNs) and impaired lithium chloride-induced CPA. Activation of dSPNs and SPNs in the indirect pathway (iSPNs) induced CPP and CPA, respectively; conversely, suppression of dSPNs or iSPNs induced CPA or CPP. In addition, activation or suppression of calretinin-expressing (CR) GABAergic interneurons did not induce place preference or aversion. These data suggest that PVC interneurons can bidirectionally determine the emotional valence through their regulation of accumbal SPN activities and raise the possibility that manipulation of PVC interneuron activity may have the potential to alter emotional valence and treat related mental disorders.
Figure 1. Optogenetic activation of ChR2 in accumbal PVC interneurons increased Fos mRNA expression in dSPNs in the NAcc.
To address the roles of PVC interneurons in the regulation of accumbal output pathways and LiCl-induced place aversion, using PV::Cre mice combined with optogenetic and chemogenetic tools, the results suggested that PVC interneurons can bidirectionally determine the emotional valence through their regulation of accumbal SPN activities and raise the possibility that manipulation of PVC interneuron activity may have the potential to alter emotional valence and treat related mental disorders.
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