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Incerta-thalamic circuit controls nocifensive behavior via ca
AAV-taCasp3 was used for ZIv PV+ neuronal selective ablation. For monosynaptic retrograde tracing. Rabies virus-mediated retrograde tracing (From BrainVTA) were used to examine the ZIv-Po connection.
The viruses used in this article From BrainVTA are in the table below
RV  R01002 RV-ENVA-DsRed
Tracing Helper  PT-0165 AAV2/9-DIO-TVA-EGFP
 PT-0023 AAV2/9-DIO-RG
Pub Date: 2019-06-04,  DOI: 10.1016/j.neuron.2020.04.027 Email: [email protected]
HaoWang, Ping Dong, Chao He, Xiao-Yang Feng, Yue Huang, Wei-Wei Yang, Hua-Jing Gao, Xiao-Fan Shen, Shan Lin, Shu-Xia Cao, Hong Lian, Jiadong Chen, Min Yan, and Xiao-Ming Li
Pain is a source of substantial discomfort. Abnormal activity in both the zona incerta (ZI) and posterior complex of the thalamus (Po) are implicated in neuropathic pain, but their exact roles remain unclear. In particular, the precise cell types andmolecularmechanisms of the ZI-Po circuit that regulate nociception are largely uncharacterized. Here, we found that parvalbumin (PV)-positive neuronal projections fromthe ventral ZI (ZIv) to the Po (ZIv-Po) are critical for promoting nocifensive behaviors, whereas selectively inhibiting ZIv-Po activity reduces nocifensive withdrawal responses. Furthermore, cannabinoid type 1 receptors (CB1Rs) are expressed specifically at ZIv-Po axon terminals in this circuit, and cannabinoids attenuate nocifensive responses through presynaptic inhibition. Selective inhibition of the ZIv-Po circuit or administration of cannabinoids into the Po are sufficient to ameliorate pathological pain. These findings identify the critical role of the ZIv-Po circuit and its modulation by endocannabinoids in controlling nocifensive behaviors.

Fig1. Using AAV-GCaMP6s and RV to study the activity of ZIv PV+ neurons
To reveal the underlying mechanisms of ZI and Po in neuropathic pain,using  virus tracing tools(Some are From BrainVTA),situ hybridization and single-cell PCR technologies, the researchers indicate that manipulation of the ZIv-Po circuit has an effect on both nocifensive behaviors and affective aspects of pain.

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